Journal: PLoS ONE

Article Title: Effect of Helicobacter pylori infection on the link between GLP-1 expression and motility of the gastrointestinal tract

doi: 10.1371/journal.pone.0177232

Figure Lengend Snippet: (A) Immunostaining of PAX6 in the colonic mucosa of mice with H . pylori infection. PAX6 is expressed in the nuclei of colonic epithelial cells (arrows) and the number of cells expressing it is increased relative to control mice without H . pylori infection. Double immunostaining showing co-expression of GLP-1 (green) and PAX6 (red) in a colonic epithelial cell. (B) Serial counts of PAX6-posiitve cells in the colonic mucosa of mice with H . pylori infection. (C) Correlation between the numbers of PAX6- and GLP-1-positive cells. ○, control without H . pylori infection; ●, H . pylori -infected mice. All the results are expressed as the mean ± SE. Significantly greater than control at start of the experiment: * P <0.05.

Article Snippet: Immunohistochemical stainings for GLP-1 and paired box protein-6 (PAX6) were performed with an Envision Kit (Dako, Kyoto, Japan) according to the manufacturer’s protocol [ ], using anti-GLP-1 antibody (Abcam, Cambridge, UK; dilution 1:500) and anti-PAX6 antibody (Merck KGaA, Darmstadt, Germany; dilution 1:500).

Techniques: Immunostaining, Infection, Expressing, Control, Double Immunostaining

Journal: PLoS ONE

Article Title: Effect of Helicobacter pylori infection on the link between GLP-1 expression and motility of the gastrointestinal tract

doi: 10.1371/journal.pone.0177232

Figure Lengend Snippet: (A) Immunostaining of GLP-1 and PAX6 in the colonic mucosa of mice infected with H . pylori , and after H . pylori eradication. Arrows indicate PAX6-positive cells. Numbers of cells expressing GLP-1 (B) and PAX6 (C) in the colonic mucosa of mice infected with H . pylori , and after H . pylori eradication. Data are presented as medians and interquartile range (n = 5 in each group). Significantly different between two groups: * P <0.05. NS, not significant.

Article Snippet: Immunohistochemical stainings for GLP-1 and paired box protein-6 (PAX6) were performed with an Envision Kit (Dako, Kyoto, Japan) according to the manufacturer’s protocol [ ], using anti-GLP-1 antibody (Abcam, Cambridge, UK; dilution 1:500) and anti-PAX6 antibody (Merck KGaA, Darmstadt, Germany; dilution 1:500).

Techniques: Immunostaining, Infection, Expressing

Journal: PLoS ONE

Article Title: Effect of Helicobacter pylori infection on the link between GLP-1 expression and motility of the gastrointestinal tract

doi: 10.1371/journal.pone.0177232

Figure Lengend Snippet: Correlation between gastrointestinal transit time and (A) colonic expression of GLP-1 or (B) PAX6. (C) Correlation between GLP-1 and PAX6 expression in the colon. White dots, controls; black dots, infected mice; gray dots, mice after eradication treatment.

Article Snippet: Immunohistochemical stainings for GLP-1 and paired box protein-6 (PAX6) were performed with an Envision Kit (Dako, Kyoto, Japan) according to the manufacturer’s protocol [ ], using anti-GLP-1 antibody (Abcam, Cambridge, UK; dilution 1:500) and anti-PAX6 antibody (Merck KGaA, Darmstadt, Germany; dilution 1:500).

Techniques: Expressing, Infection

Journal: Nature Communications

Article Title: Pogz deficiency leads to transcription dysregulation and impaired cerebellar activity underlying autism-like behavior in mice

doi: 10.1038/s41467-020-19577-0

Figure Lengend Snippet: a Comparison of the body size of Pogz cKO −/− and control mice littermates (P11). b Body weight in four time points (P13, P21, P40, P60). Repeated measures ANOVA; n = 16 control (blue), 7 cKO +/− (gray), 7 cKO −/− (red), P = 1.1 × 10 −15 . c Brain weight at P11. n = 5 control, 5 cKO +/− , and 3 cKO −/ − . d Coronal sections of embryonic cerebral cortex (E15.5) stained with anti-Pax6 and Tbr2 antibodies. Scale bar = 50 μm. VZ ventricular zone, SVZ subventricular zone. e , f Quantification of the thickness of the Tbr2+ and Pax6+ layers. Values are proportion relative to control mice. n = 5 control, 9 cKO +/− , 9 cKO −/− in four independent experiments. g , h Coronal sections of cerebral cortices (E15.5) stained with DAPI and antibody against phosphorylated histone 3 (pHH3). Scale bar = 100 μm. h Quantification of the number of the pHH3+ cells. Values are proportion relative to control mice. n = 5 control, 9 cKO +/− , 11 cKO −/− in two independent experiments. i , j Effects of Pogz-silencing on cell-cycle exit. i Differentiated neurons are EdU/GFP-double-positive while EdU/Ki67/GFP-triple-positive cells maintain progenitor potency. Arrowheads indicate triple-positive cells. Scale bar = 10 µm. At the bottom right is the scheme of the cell-cycle exit assay. j Quantification of EdU/Ki67/GFP-triple-positive cells among GFP/EdU double-positive ones (Cell exit index). Two sections were used per brain (more than 1600 cells were analyzed in each condition). n = 10 control, 9 sh-Pogz#1 (sh#1), 10 sh-Pogz#2 (sh#2) from two in utero independent experiments. k − m Reduced adult neurogenesis associated with Pogz deficiency. Quantification of either k DCX-positive cells, l Ki67-positive cells, m BrdU-positive cells in the dentate gyrus, 21 days post injection. Numbers are proportion relative to control mice. n = 6 control, 6 cKO +/− , 3 cKO −/− . The P values at the top of the plots are for the association between the quantitative measurements and the number of intact Pogz alleles calculated with a linear regression model. Pairwise comparisons between genotypes was calculated by two-tailed t test and the significance is represented by: * P < 0.05; ** P < 0.01; *** P < 0.001; n.s. not significant. Quantitative data are mean ± SEM.

Article Snippet: Rabbit anti-POGZ (ab171934 1:500); Mouse anti-NeuN (MAB377 1:400); Mouse anti-CUTL1/CUX1 (ab54583 1:500); Rat anti-CTIP2 (ab18465 1:500); Rabbit anti-TBR1 (Merck AB10554 1:400); Rabbit anti-Ki67 (ab15580 1:1000); Rat anti-BrdU (ab6326 1:250) Mouse anti-DCX (sc271390 1:200); Mouse anti-SOX2 (sc17320 1:1000); Goat anti-NeuroD (sc1804 1:1000); Rabbit anti-PROX1 (Merck AB5475 1:1000); Rabbit anti-PAX6 (Merck AB2237 1:500); Mouse anti-CNPase (ab6319 1:1000); Mouse anti-Calbindin (Merck C9848 1:1000); Rabbit anti-GFAP (ab7260 1:1000); goat anti-rabbit Alexa fluor 568 (Invitrogen A-11011 1:200); goat anti-mouse Alexa fluor 488 (Invitrogen A-11001 1:200).

Techniques: Staining, In Utero, Injection, Two Tailed Test